Microarrays Research Today is a free monthly online journal that collates and summarizes the latest research about Microarrays, including details on experiments, designs, statistics, analysis, software. | ||||||||
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Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology.Vigneswaran N, Wu J, Sacks P, Gilcrease M, Zacharias W Department of Diagnostic Sciences, University of Texas-Houston Dental Branch, Houston, TX, USA. BACKGROUND: To identify common gene expression patterns among two uniquely matched pairs of primary and metastatic oral squamous cell carcinoma (OSCC) cell lines derived from the same two patient donors. METHODS: Two pairs of cell lines derived from the primary tumors and lymph node metastases of the same two patients were used to obtain microarray-based gene expression profiles. Reverse transcriptase-polymerase chain reaction and immunohistochemistry were used to confirm observed changes for some of the candidate genes. RESULTS: Approximately 50% of the genes profiled were expressed in all four cell lines. Cluster analysis identified a group of 17 genes whose expression correlated inversely with metastatic progression. Only 10 common genes were differentially expressed in both pairs of primary and metastatic cells. A group of 28 highly expressed genes was common for both metastatic cell lines, among them some of the known metastasis-related genes such as laminin receptor, thymosin beta-4 and beta-10 and metallopanstimulin. CONCLUSIONS: Groups of presumed metastasis-related genes are highly heterogeneous and vary significantly between the two patients. Thus, it is unlikely that the metastatic phenotype of these OSCC cells is acquired by de-regulation of a single gene or a group of few genes. Most likely, multiple combinations of differentially expressed genes are involved in facilitating metastatic spread of these oral carcinoma cell lines. Published 11 January 2005 in J Oral Pathol Med, 34(2): 77-86.
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