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Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing.

Girish A, Sun H, Yeo DS, Chen GY, Chua TK, Yao SQ

Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.

Published 2 May 2005 in Bioorg Med Chem Lett, 15(10): 2447-51.
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