Microarrays Research Today is a free monthly online journal that collates and summarizes the latest research about Microarrays, including details on experiments, designs, statistics, analysis, software.

Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Carter MG, Sharov AA, VanBuren V, Dudekula DB, Carmack CE, Nelson C, Ko MS

Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA. cartermar@mail.nih.gov

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

Published 6 July 2005 in Genome Biol, 6(7): R61.
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