Microarrays Research Today is a free monthly online journal that collates and summarizes the latest research about Microarrays, including details on experiments, designs, statistics, analysis, software.

Quality assessment of microarray experiments.

Carter DE, Robinson JF, Allister EM, Huff MW, Hegele RA

London Regional Genomics Centre, Robarts Research Institute, London, Ontario, Canada N6A 5K8.

OBJECTIVES: We describe a quality assurance procedure to maximize the value of oligonucleotide microarray expression profiles. DESIGN, METHODS, AND RESULTS: Background microarray noise was 82.2+/-54.5 and 51.8+/-12.4 units, respectively, before and after enacting the program (P<0.0001). We also noted improved concordance of microarray expression fold-changes for selected genes with results of RT-PCR validation. CONCLUSIONS: This multi-step procedure, including quantification of RNA sample degradation and detection of outlier data points, has increased data quality from our microarray facility.

Published 12 July 2005 in Clin Biochem, 38(7): 639-42.
Full-text of this article is available online (may require subscription).

© 2004-2008 Microarrays Research Today. All Rights Reserved.