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MASQOT-GUI: spot quality assessment for the two-channel microarray platform.

Bylesjö M, Sjödin A, Eriksson D, Antti H, Moritz T, Jansson S, Trygg J

Research group for Chemometrics, Department of Chemistry, Umeå University SE-901 87 Umeå, Sweden. max.bylesjo@chem.umu.se

MASQOT-GUI provides an open-source, platform-independent software pipeline for two-channel microarray spot quality control. This includes gridding, segmentation, quantification, quality assessment and data visualization. It hosts a set of independent applications, with interactions between the tools as well as import and export support for external software. The implementation of automated multivariate quality control assessment, which is a unique feature of MASQOT-GUI, is based on the previously documented and evaluated MASQOT methodology. Further abilities of the application are outlined and illustrated. AVAILABILITY: MASQOT-GUI is Java-based and licensed under the GNU LGPL. Source code and installation files are available for download at http://masqot-gui.sourceforge.net/

Published 11 October 2006 in Bioinformatics, 22(20): 2554-5.
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