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Fabrication and application of carbohydrate microarray for analyzing human serum antibody-carbohydrate interaction.

Huang GL, Mei XY, Zhang HX, Wang PG

State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, Shandong, 250100, People’s Republic of China, hgl226@126.com.

We introduced a strategy for preparing a carbohydrate microarray and demonstrated its utility for characterizing carbohydrate binding and activities. We isolated the lipopolysaccharide (LPS) components from different bacteria and explored the possibility of immobilizing these glycoconjugates on a high-binding polystyrene plate. Carbohydrate-specific combination was examined by observing the binding of the blood group B analogic LPS O-polysaccharide from Escherichia coli on the high-binding polystyrene plate and anti-B from a broad spectra antibody of human blood serum. Strong binding of antibodies was screened, as it was evident that relative response value is two times higher than control. The hybridization results indicated that this method is a reliable technique for the detection of human intestinal bacteria and is expected to be applied in diagnostics and seroepidemiology.

Published 17 November 2006 in Appl Microbiol Biotechnol.
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