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Evaluation of differential gene expression in susceptible and resistant clinical isolates of Klebsiella pneumoniae by DNA microarray analysis.

Doménech-Sánchez A, Benedí VJ, Martínez-Martínez L, Albertí S

Departamento de Biología and Institut Universitari d'Investigació en Ciències de la Salut, Universidad de las Islas Baleares, Palma de Mallorca, Spain.

DNA microarray technology was used to evaluate differential gene expression in a susceptible Klebsiella pneumoniae isolate and a resistant clinical derivative. Nineteen genes were up-regulated in the resistant isolate when compared with the susceptible isolate. An ABC transporter-related gene, ycjV, was strongly over-expressed, suggesting the existence of a novel active efflux mechanism. Approximately half of the up-regulated genes coded for ribosomal proteins, or proteins involved in tRNA metabolism. Among 33 downregulated genes, almost one-third were related to nitrogen metabolism. A possible role of fitness in the development of antimicrobial resistance is suggested.

Published 2 August 2006 in Clin Microbiol Infect, 12(9): 936-40.
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