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DNA microarray for detection of antibiotic resistance determinants in Bacillus anthracis and closely related Bacillus cereus.

Antwerpen MH, Schellhase M, Ehrentreich-Förster E, Bier F, Witte W, Nübel U

Robert Koch-Institut, Burgstrasse 37, 38855 Wernigerode, Germany.

We developed a multiplex PCR for amplification of ten genes involved in resistance to ciprofloxacin, doxycycline, rifampin, and vancomycin in Bacillus anthracis and closely related Bacillus cereus. Enzymatic labelling of PCR products followed by hybridization to oligonucleotide probes on a DNA microarray enabled simultaneous detection of resistance genes tetK, tetL, tetM, tetO, vanA, and vanB and resistance-mediating point mutations in genes gyrA, gyrB, parC, and rpoB. The presented assay allows detection of clinically relevant antibiotic resistance determinants within 4h and can be used as a time-saving tool supporting conventional culture-based diagnostics.

Published 16 January 2007 in Mol Cell Probes, 21(2): 152-60.
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