Microarrays Research Today is a free monthly online journal that collates and summarizes the latest research about Microarrays, including details on experiments, designs, statistics, analysis, software.

A novel tissue array technique for high-throughput tissue microarray analysis - microarray groups.

Jiang HY, Zhang XF, Liu L, Li HL, Zhao T

Department of General Surgery, Northern Hospital, No. 83, Wenhua Road, Shenhe District, Shenyang, 110016, Liaoning Province, China, huiyongjsy@yahoo.com.cn.

Tissue microarrays are ordered arrays of hundreds to thousands of tissue cores in a single paraffin block. We invented a novel method to make a high-throughput microarray group. Conventional smaller tissue microarrays were made first and then sectioned. Separate paraffin films were arrayed orderly onto a regular-sized glass slide to form a larger microarray group. Sections were not floated in a water bath but, rather, were cut singly using conventional microtome, arrayed orderly onto the glass slide with forceps instead of using a tape-based tissue transfer system, and then unfolded with warm water (46 degrees C) using a micropipette. This not only lowers the difficulty in sectioning but the overall tissue disks can be included in the same section. A microarray group of 2,534 small disks (theoretically, 2,560 disks can be made; 26 fell off during the procedure), the most up to now, was successfully made and may be used in immunohistochemistry, mRNA in situ hybridization, and flourescent in situ hybridization.

Published 7 June 2007 in In Vitro Cell Dev Biol Anim, 43(3): 109-12.
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