Microarrays Research Today is a free monthly online journal that collates and summarizes the latest research about Microarrays, including details on experiments, designs, statistics, analysis, software.

Microarray based Raman spectroscopic detection with gold nanoparticle probes.

Li T, Guo L, Wang Z

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; School of Chemistry, Northeast Normal University, Changchun 130022, China.

A microarray approach based on surface-enhanced Raman spectroscopic (SERS) was developed for detection of spotted peptide, peptide-protein or protein-antibody interaction. The procedure involves the attachment of peptide-capped gold nanoparticles followed by silver deposition for signal enhancement. The attachment of the gold nanoparticles is achieved by standard avidin-biotin chemistry. The well-known biomolecular recognition pairs, IgG/protein A and biotin/avidin, were used to demonstrate proof-of-concept of the SERS assay. Detection limits of 10 and 100fg per microarray spot were obtained respectively for the peptide and protein arrays. For the protein in solution, a limit of 0.1mug/mL is reported. Furthermore, enzyme activity of the kinase (PKA) is also detected with high specificity for an established peptide substrate (kemptide) on the microarray spots.

Published 4 February 2008 in Biosens Bioelectron, 23(7): 1125-30.
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