Microarrays Research Today is a free monthly online journal that collates and summarizes the latest research about Microarrays, including details on experiments, designs, statistics, analysis, software.

Oligonucleotide Microarray Analysis of Gene Expression Profiles followed by Real-Time Reverse-Transcriptase Polymerase Chain Reaction Assay in Chronic Active Epstein-Barr Virus Infection.

Ito Y, Shibata-Watanabe Y, Ushijima Y, Kawada JI, Nishiyama Y, Kojima S, Kimura H

1 Department of Pediatrics and 2 Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Chronic active Epstein-Barr virus infection (CAEBV) is characterized by recurrent infectious mononucleosis-like symptoms and has high mortality and morbidity. To clarify the mechanisms of CAEBV, the gene-expression profiles of peripheral blood obtained from patients with CAEBV were investigated. Twenty genes were differentially expressed in 4 patients with CAEBV. This microarray result was verified using a real-time reverse-transcriptase polymerase chain reaction assay in a larger group of patients with CAEBV. Eventually, 3 genes were found to be significantly upregulated: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5. These genes may be associated with the inflammatory reaction or with cell proliferation.

Published 11 February 2008 in J Infect Dis.
Full-text of this article is available online (may require subscription).

© 2004-2008 Microarrays Research Today. All Rights Reserved.